› 4q2g

X-ray diffraction
3.4Å resolution

CRYSTAL STRUCTURE OF AN INTRAMEMBRANE CDP-DAG SYNTHETASE CENTRAL FOR PHOSPHOLIPID BIOSYNTHESIS (S200C/S223C, inactive mutant)

Released:
DOI: 10.2210/pdb4q2g/pdb
PDB entry 4q2g coloured by chain, front view.
PDB entry 4q2g coloured by chain, side view.
PDB entry 4q2g coloured by chain, top view.
Source organism: Thermotoga maritima MSB8
Primary publication:
Structure and mechanism of an intramembrane liponucleotide synthetase central for phospholipid biosynthesis.
Liu X, Yin Y, Wu J, Liu Z
Nat Commun 5 4244 (2014)
PMID: 24968740

Function and Biology Details

Reaction catalysed: 
CTP + phosphatidate = diphosphate + CDP-diacylglycerol
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194783 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphatidate cytidylyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 290 amino acids
Theoretical weight: 32.33 KDa
Source organism: Thermotoga maritima MSB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9X1B7 (Residues: 1-270; Coverage: 100%)
Gene names: TM_1397, cdsA
Sequence domains: Cytidylyltransferase family

Ligands and Environments

3 bound ligands:
Ligand HG 4 x HG
Ligand MG 2 x MG
Ligand BNG 2 x BNG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P6522
Unit cell:
a: 141.41Å b: 141.41Å c: 197.76Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.291 0.291 0.315
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Reprogramming of fatty acid metabolism in cancer.
Koundouros et al. (2020)
6 more

3 mentions without citation

The Blueprint of a Minimal Cell: MiniBacillus.
Reuß et al. (2016)
2 more