4pw7

X-ray diffraction
2Å resolution

structure of UHRF2-SRA in complex with a 5mC-containing DNA

Released:
Source organism: Homo sapiens

Function and Biology Details

Reaction catalysed:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-117990 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
E3 ubiquitin-protein ligase UHRF2 Chains: A, B, E, F
Molecule details ›
Chains: A, B, E, F
Length: 230 amino acids
Theoretical weight: 25.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96PU4 (Residues: 419-648; Coverage: 29%)
Gene names: NIRF, RNF107, UHRF2
Sequence domains: SAD/SRA domain
Structure domains: SRA-YDG
5mC-containing DNA1 Chains: C, G
Molecule details ›
Chains: C, G
Length: 12 nucleotides
Theoretical weight: 3.71 KDa
Source organism: Homo sapiens
Expression system: Not provided
5mC-containing DNA2 Chains: D, H
Molecule details ›
Chains: D, H
Length: 12 nucleotides
Theoretical weight: 3.59 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: BSRF BEAMLINE 1W2B
Spacegroup: P1
Unit cell:
a: 42.331Å b: 83.694Å c: 83.686Å
α: 89.97° β: 89.94° γ: 90.02°
R-values:
R R work R free
0.166 0.163 0.211
Expression systems:
  • Escherichia coli
  • Not provided