› 4ptv

X-ray diffraction
1.85Å resolution

Halothermothrix orenii beta-glucosidase A, thiocellobiose complex

Released:
DOI: 10.2210/pdb4ptv/pdb
PDB entry 4ptv coloured by chain, front view.
PDB entry 4ptv coloured by chain, side view.
PDB entry 4ptv coloured by chain, top view.
Source organism: Halothermothrix orenii H 168
Primary publication:
Biochemical and structural characterization of a thermostable β-glucosidase from Halothermothrix orenii for galacto-oligosaccharide synthesis.
Hassan N, Nguyen TH, Intanon M, Kori LD, Patel BK, Haltrich D, Divne C, Tan TC
Appl Microbiol Biotechnol 99 1731-44 (2015)
PMID: 25173693

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110674 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Beta-glucosidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 452 amino acids
Theoretical weight: 52.29 KDa
Source organism: Halothermothrix orenii H 168
Expression system: Escherichia coli
UniProt:
  • Canonical: B8CYA8 (Residues: 1-451; Coverage: 100%)
Gene name: Hore_15280
Sequence domains: Glycosyl hydrolase family 1
Structure domains: Glycosidases

Ligands and Environments

Carbohydrate polymer : NEW Components: SGC, BGC
Ligand SGC 2 x SGC
Ligand BGC 2 x BGC
2 bound ligands:
Ligand CS 2 x CS
Ligand PE4 2 x PE4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P212121
Unit cell:
a: 87.753Å b: 99.068Å c: 108.09Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.196 0.227
Expression system: Escherichia coli

Quick links

Citations

22 citation in other articles

Overexpression and characterization of a glucose-tolerant β-glucosidase from T. aotearoense with high specific activity for cellobiose.
Yang et al. (2015)
21 more

6 mentions without citation

A mechanism of glucose tolerance and stimulation of GH1 β-glucosidases.
Yang et al. (2015)
5 more