› 4pop

X-ray diffraction
2.2Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106937 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thiamine transporter ThiT Chains: A, B
Molecule details ›
Chains: A, B
Length: 192 amino acids
Theoretical weight: 21.23 KDa
Source organism: Lactococcus lactis subsp. cremoris MG1363
Expression system: Lactococcus lactis
UniProt:
  • Canonical: A2RI47 (Residues: 1-182; Coverage: 100%)
Gene names: llmg_0334, thiT
Sequence domains: Thiamine transporter protein (Thia_YuaJ)
Structure domains: Arp2/3 complex 21 kDa subunit ARPC3

Ligands and Environments

9 bound ligands:
Ligand 2VY 2 x 2VY
Ligand PG0 3 x PG0
Ligand BNG 5 x BNG
Ligand PEG 21 x PEG
Ligand PG4 9 x PG4
Ligand 1PE 3 x 1PE
Ligand PGE 1 x PGE
Ligand P6G 1 x P6G
Ligand P33 1 x P33
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: C2
Unit cell:
a: 62.61Å b: 84.12Å c: 127.15Å
α: 90° β: 95.13° γ: 90°
R-values:
R R work R free
0.199 0.199 0.248
Expression system: Lactococcus lactis

Quick links

Citations

2 review citations

ECF-Type ATP-Binding Cassette Transporters.
Rempel et al. (2019)
1 more

2 mentions without citation

Elevator-type mechanisms of membrane transport.
Garaeva et al. (2020)
1 more