› 4pk4

X-ray diffraction
2.78Å resolution

Myosin VI motor domain in the PPS state - from a Pi release state crystal, space group P212121 after long soaking with PO4

Released:
DOI: 10.2210/pdb4pk4/pdb
PDB entry 4pk4 coloured by chain, front view.
PDB entry 4pk4 coloured by chain, side view.
PDB entry 4pk4 coloured by chain, top view.
Source organism: Sus scrofa
Primary publication:
How actin initiates the motor activity of Myosin.
Llinas P, Isabet T, Song L, Ropars V, Zong B, Benisty H, Sirigu S, Morris C, Kikuti C, Safer D, Sweeney HL, Houdusse A
Dev Cell 33 401-12 (2015)
PMID: 25936506

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123361 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Unconventional myosin-VI Chain: A
Molecule details ›
Chain: A
Length: 788 amino acids
Theoretical weight: 89.85 KDa
Source organism: Sus scrofa
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: F1RQI7 (Residues: 2-789; Coverage: 62%)
Gene name: MYO6
Sequence domains: Myosin head (motor domain)
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ADP 1 x ADP
Ligand MG 1 x MG
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P212121
Unit cell:
a: 93.59Å b: 94.79Å c: 101.94Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.187 0.259
Expression system: Spodoptera frugiperda

Quick links

Citations

16 review citations

How Myosin Generates Force on Actin Filaments.
Houdusse et al. (2016)
15 more

3 mentions without citation

How actin initiates the motor activity of Myosin.
Llinas et al. (2015)
2 more