› 4pet

X-ray diffraction
1.9Å resolution

CRYSTAL STRUCTURE OF A TRAP PERIPLASMIC SOLUTE BINDING PROTEIN FROM COLWELLIA PSYCHRERYTHRAEA (CPS_0129, TARGET EFI-510097) WITH BOUND CALCIUM AND PYRUVATE

Released:
DOI: 10.2210/pdb4pet/pdb
PDB entry 4pet coloured by chain, front view.
PDB entry 4pet coloured by chain, side view.
PDB entry 4pet coloured by chain, top view.
Source organism: Colwellia psychrerythraea 34H
Primary publication:
Experimental strategies for functional annotation and metabolism discovery: targeted screening of solute binding proteins and unbiased panning of metabolomes.
Vetting MW, Al-Obaidi N, Zhao S, San Francisco B, Kim J, Wichelecki DJ, Bouvier JT, Solbiati JO, Vu H, Zhang X, Rodionov DA, Love JD, Hillerich BS, Seidel RD, Quinn RJ, Osterman AL, Cronan JE, Jacobson MP, Gerlt JA, Almo SC
Biochemistry 54 909-31 (2015)
PMID: 25540822

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175375 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Extracellular solute-binding protein, family 7 Chains: A, B
Molecule details ›
Chains: A, B
Length: 357 amino acids
Theoretical weight: 41.63 KDa
Source organism: Colwellia psychrerythraea 34H
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q48AL6 (Residues: 29-362; Coverage: 100%)
Gene name: CPS_0129
Sequence domains: Bacterial extracellular solute-binding protein, family 7
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand CL 1 x CL
Ligand PYR 2 x PYR
Ligand CA 2 x CA
1 modified residue:
Ligand MSE 42 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P212121
Unit cell:
a: 55.827Å b: 70.249Å c: 154.927Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.156 0.154 0.207
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

13 review citations

Enzyme Function Initiative-Enzyme Similarity Tool (EFI-EST): A web tool for generating protein sequence similarity networks.
Gerlt et al. (2015)
12 more

1 mention without citation

Experimental strategies for functional annotation and metabolism discovery: targeted screening of solute binding proteins and unbiased panning of metabolomes.
Vetting et al. (2015)