› 4pbb

X-ray diffraction
3.46Å resolution

The 5-Hydroxymethylcytosine-Specific Restriction Enzyme AbaSI

Released:
DOI: 10.2210/pdb4pbb/pdb
PDB entry 4pbb coloured by chain, front view.
PDB entry 4pbb coloured by chain, side view.
PDB entry 4pbb coloured by chain, top view.
Source organism: Acinetobacter baumannii SDF
Primary publication:
Structure of 5-hydroxymethylcytosine-specific restriction enzyme, AbaSI, in complex with DNA.
Horton JR, Borgaro JG, Griggs RM, Quimby A, Guan S, Zhang X, Wilson GG, Zheng Y, Zhu Z, Cheng X
Nucleic Acids Res 42 7947-59 (2014)
PMID: 24895434

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109252 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uncharacterized protein AbaSI Chains: A, B
Molecule details ›
Chains: A, B
Length: 321 amino acids
Theoretical weight: 37.67 KDa
Source organism: Acinetobacter baumannii SDF
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B0VN39 (Residues: 1-321; Coverage: 100%)
Gene name: ABSDF3356
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 5 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 67.428Å b: 92.352Å c: 180.846Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.243 0.24 0.28
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

DNA Base Flipping: A General Mechanism for Writing, Reading, and Erasing DNA Modifications.
Hong et al. (2016)
19 other articles

2 mentions without citation

Structure of 5-hydroxymethylcytosine-specific restriction enzyme, AbaSI, in complex with DNA.
Horton et al. (2014)
1 more