› 4p7l

X-ray diffraction
1.8Å resolution

Structure of Escherichia coli PgaB C-terminal domain, P212121 crystal form

Released:
DOI: 10.2210/pdb4p7l/pdb
PDB entry 4p7l coloured by chain, front view.
PDB entry 4p7l coloured by chain, side view.
PDB entry 4p7l coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Modification and periplasmic translocation of the biofilm exopolysaccharide poly-β-1,6-N-acetyl-D-glucosamine.
Little DJ, Li G, Ing C, DiFrancesco BR, Bamford NC, Robinson H, Nitz M, Pomès R, Howell PL
Proc Natl Acad Sci U S A 111 11013-8 (2014)
PMID: 24994902

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160020 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Poly-beta-1,6-N-acetyl-D-glucosamine N-deacetylase Chain: A
Molecule details ›
Chain: A
Length: 367 amino acids
Theoretical weight: 42.44 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P75906 (Residues: 310-672; Coverage: 56%)
Gene names: JW5142, b1023, pgaB, ycdR
Sequence domains: Hypothetical glycosyl hydrolase family 13
Structure domains: Glycosidases

Ligands and Environments

2 bound ligands:
Ligand EDO 1 x EDO
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 41.764Å b: 78.213Å c: 97.924Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.145 0.142 0.189
Expression system: Escherichia coli BL21

Quick links

Citations

6 review citations

Potential targets for next generation antimicrobial glycoconjugate vaccines.
Micoli et al. (2018)
5 more

2 mentions without citation

Modification and periplasmic translocation of the biofilm exopolysaccharide poly-β-1,6-N-acetyl-D-glucosamine.
Little et al. (2014)
1 more