› 4ot1

X-ray diffraction
2.11Å resolution

Structural Basis for the Recognition of Human Cytomegalovirus Glycoprotein B by the Neutralizing Human Antibody SM5-1

Released:
DOI: 10.2210/pdb4ot1/pdb
PDB entry 4ot1 coloured by chain, front view.
PDB entry 4ot1 coloured by chain, side view.
PDB entry 4ot1 coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for the recognition of human cytomegalovirus glycoprotein B by a neutralizing human antibody.
Spindler N, Diestel U, Stump JD, Wiegers AK, Winkler TH, Sticht H, Mach M, Muller YA
PLoS Pathog 10 e1004377 (2014)
PMID: 25299639

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-210537 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Envelope glycoprotein B Chain: A
Molecule details ›
Chain: A
Length: 129 amino acids
Theoretical weight: 14.43 KDa
Source organism: Human herpesvirus 5 strain Towne
Expression system: Escherichia coli
UniProt:
  • Canonical: P13201 (Residues: 114-133, 344-438; Coverage: 13%)
Gene names: UL55, gB
Sequence domains:
Structure domains: PH-domain like
SM5-1 Fab Heavy Chain Chain: H
Molecule details ›
Chain: H
Length: 234 amino acids
Theoretical weight: 24.88 KDa
Source organism: Homo sapiens
Expression system: Not provided
Structure domains: Immunoglobulins
SM5-1 Fab Light Chain Chain: L
Molecule details ›
Chain: L
Length: 215 amino acids
Theoretical weight: 22.57 KDa
Source organism: Homo sapiens
Expression system: Not provided
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand EDO 3 x EDO
Ligand EPE 1 x EPE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P21
Unit cell:
a: 43.071Å b: 69.411Å c: 100.991Å
α: 90° β: 99.68° γ: 90°
R-values:
R R work R free
0.174 0.171 0.231
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

5 review citations

Human antibody technology and the development of antibodies against cytomegalovirus.
Ohlin et al. (2015)
4 more

7 mentions without citation

Crystal Structure of the Human Cytomegalovirus Glycoprotein B.
Burke et al. (2015)
6 more