› 4opn

X-ray diffraction
2.1Å resolution

Crystal structure of mouse glyoxalase I complexed with mAH

Released:
DOI: 10.2210/pdb4opn/pdb
PDB entry 4opn coloured by chain, front view.
PDB entry 4opn coloured by chain, side view.
PDB entry 4opn coloured by chain, top view.
Source organism: Mus musculus
Entry authors: Zhai J, Zhang L, Yuan M, Zhang H

Function and Biology Details

Reaction catalysed: 
(R)-S-lactoylglutathione = glutathione + methylglyoxal
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189993 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lactoylglutathione lyase Chains: A, B
Molecule details ›
Chains: A, B
Length: 184 amino acids
Theoretical weight: 20.84 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9CPU0 (Residues: 1-184; Coverage: 100%)
Gene name: Glo1
Sequence domains: Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Structure domains: 2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1

Ligands and Environments


Cofactor: Ligand ZBF 2 x ZBF
1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: OXFORD DIFFRACTION ENHANCE ULTRA
Spacegroup: P21
Unit cell:
a: 41.559Å b: 65.084Å c: 64.885Å
α: 90° β: 101.29° γ: 90°
R-values:
R R work R free
0.18 0.177 0.231
Expression system: Escherichia coli

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