› 4odr

X-ray diffraction
1.93Å resolution

Structure of SlyD delta-IF from Thermus thermophilus in complex with FK506

Released:
DOI: 10.2210/pdb4odr/pdb
PDB entry 4odr coloured by chain, front view.
PDB entry 4odr coloured by chain, side view.
PDB entry 4odr coloured by chain, top view.
Source organisms:
Primary publication:
Molecular insights into substrate recognition and catalytic mechanism of the chaperone and FKBP peptidyl-prolyl isomerase SlyD.
Quistgaard EM, Weininger U, Ural-Blimke Y, Modig K, Nordlund P, Akke M, Löw C
BMC Biol 14 82 (2016)
PMID: 27664121

Function and Biology Details

Reaction catalysed: 
Peptidylproline (omega=180) = peptidylproline (omega=0)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158811 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peptidyl-prolyl cis-trans isomerase; Peptidyl-prolyl cis-trans isomerase FKBP1A Chains: A, B
Molecule details ›
Chains: A, B
Length: 110 amino acids
Theoretical weight: 12.18 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: Q5SLE7 (Residues: 1-64, 127-149; Coverage: 58%)
  • Canonical: P62942 (Residues: 85-98; Coverage: 13%)
Gene names: FKBP1, FKBP12, FKBP1A, TTHA0346
Sequence domains: FKBP-type peptidyl-prolyl cis-trans isomerase
Structure domains: Chitinase A; domain 3

Ligands and Environments

5 bound ligands:
Ligand FK5 2 x FK5
Ligand ZN 3 x ZN
Ligand CL 2 x CL
Ligand GOL 6 x GOL
Ligand ACT 3 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P43212
Unit cell:
a: 72.5Å b: 72.5Å c: 178.96Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.153 0.153 0.17
Expression system: Escherichia coli

Quick links

Citations

11 citation in other articles

Type 9 secretion system structures reveal a new protein transport mechanism.
Lauber et al. (2018)
10 more

1 mention without citation

Molecular insights into substrate recognition and catalytic mechanism of the chaperone and FKBP peptidyl-prolyl isomerase SlyD.
Quistgaard et al. (2016)