› 4nws

X-ray diffraction
2.7Å resolution

Crystal structure of PSLssmKate, a photoswitchable LSSmKate variant

Released:
DOI: 10.2210/pdb4nws/pdb
PDB entry 4nws coloured by chain, front view.
PDB entry 4nws coloured by chain, side view.
PDB entry 4nws coloured by chain, top view.
Source organism: synthetic construct
Entry authors: Malashkevich VN, Piatkevich K, Almo SC, Verkhusha V

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112213 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fluorescent protein FP480 Chains: A, B
Molecule details ›
Chains: A, B
Length: 243 amino acids
Theoretical weight: 27.62 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
UniProt:
  • Canonical: D0VX33 (Residues: 1-233; Coverage: 100%)
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand NRQ 2 x NRQ

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: I4
Unit cell:
a: 99.032Å b: 99.032Å c: 106.614Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.179 0.253
Expression system: Escherichia coli

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