› 4nu3

X-ray diffraction
1.4Å resolution

Crystal structure of mFfIBP, a capping head region swapped mutant of ice-binding protein

Released:
DOI: 10.2210/pdb4nu3/pdb
PDB entry 4nu3 coloured by chain, front view.
PDB entry 4nu3 coloured by chain, side view.
PDB entry 4nu3 coloured by chain, top view.
Source organism: Flavobacterium frigoris PS1
Primary publication:
Structure-based characterization and antifreeze properties of a hyperactive ice-binding protein from the Antarctic bacterium Flavobacterium frigoris PS1.
Do H, Kim SJ, Kim HJ, Lee JH
Acta Crystallogr D Biol Crystallogr 70 1061-73 (2014)
PMID: 24699650

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-124887 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ice-binding protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 255 amino acids
Theoretical weight: 26.34 KDa
Source organism: Flavobacterium frigoris PS1
Expression system: Escherichia coli
UniProt:
  • Canonical: H7FWB6 (Residues: 29-97, 98-108, 109-276; Coverage: 96%)
Gene name: HJ01_03463
Sequence domains: Ice-binding-like

Ligands and Environments

2 bound ligands:
Ligand SO4 4 x SO4
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: P1
Unit cell:
a: 31.681Å b: 51.459Å c: 75.23Å
α: 104.41° β: 96.85° γ: 97.67°
R-values:
R R work R free
0.225 0.223 0.25
Expression system: Escherichia coli

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Citations

7 review citations

Marine Antifreeze Proteins: Structure, Function, and Application to Cryopreservation as a Potential Cryoprotectant.
Kim et al. (2017)
6 more

2 mentions without citation

Structure and application of antifreeze proteins from Antarctic bacteria.
Muñoz et al. (2017)
1 more