› 4nc8

X-ray diffraction
2.17Å resolution

N-terminal domain of delta-subunit of RNA polymerase complexed with nickel ions

Released:
DOI: 10.2210/pdb4nc8/pdb
PDB entry 4nc8 coloured by chain, front view.
PDB entry 4nc8 coloured by chain, side view.
PDB entry 4nc8 coloured by chain, top view.
Source organism: Bacillus subtilis subsp. subtilis str. 168
Primary publication:
X-ray vs. NMR structure of N-terminal domain of δ-subunit of RNA polymerase.
Demo G, Papoušková V, Komárek J, Kadeřávek P, Otrusinová O, Srb P, Rabatinová A, Krásný L, Žídek L, Sklenář V, Wimmerová M
J Struct Biol 187 174-186 (2014)
PMID: 24937760

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-146165 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA-directed RNA polymerase subunit delta Chains: A, B
Molecule details ›
Chains: A, B
Length: 99 amino acids
Theoretical weight: 11.77 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli
UniProt:
  • Canonical: P12464 (Residues: 2-92; Coverage: 53%)
Gene names: BSU37160, rpoE
Sequence domains: HB1, ASXL, restriction endonuclease HTH domain
Structure domains: RNA polymerase, subunit delta, N-terminal domain

Ligands and Environments

1 bound ligand:
Ligand NI 2 x NI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: C2221
Unit cell:
a: 39.462Å b: 109.636Å c: 82.928Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.267 0.224 0.283
Expression system: Escherichia coli

Quick links

Citations

5 citation in other articles

In-cell architecture of an actively transcribing-translating expressome.
O'Reilly et al. (2020)
4 more

1 mention without citation

Structural biology of bacterial RNA polymerase.
Murakami KS. (2015)