› 4n7z

X-ray diffraction
2.85Å resolution

Crystal structure of human Plk4 cryptic polo box (CPB) in complex with a Cep192 N-terminal fragment

Released:
DOI: 10.2210/pdb4n7z/pdb
PDB entry 4n7z coloured by chain, front view.
PDB entry 4n7z coloured by chain, side view.
PDB entry 4n7z coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis.
Park SY, Park JE, Kim TS, Kim JH, Kwak MJ, Ku B, Tian L, Murugan RN, Ahn M, Komiya S, Hojo H, Kim NH, Kim BY, Bang JK, Erikson RL, Lee KW, Kim SJ, Oh BH, Yang W, Lee KS
Nat Struct Mol Biol 21 696-703 (2014)
PMID: 24997597

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126288 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Serine/threonine-protein kinase PLK4 Chain: A
Molecule details ›
Chain: A
Length: 229 amino acids
Theoretical weight: 26.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O00444 (Residues: 580-808; Coverage: 24%)
Gene names: PLK4, SAK, STK18
Sequence domains:
Structure domains:
Centrosomal protein of 192 kDa Chain: B
Molecule details ›
Chain: B
Length: 58 amino acids
Theoretical weight: 6.67 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8TEP8 (Residues: 201-258; Coverage: 2%)
Gene names: CEP192, KIAA1569, PP8407

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P6122
Unit cell:
a: 67.308Å b: 67.308Å c: 287.58Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.237 0.234 0.26
Expression system: Escherichia coli

Quick links

Citations

21 review citations

Centrosome function and assembly in animal cells.
Conduit et al. (2015)
20 more

4 mentions without citation

Building a ninefold symmetrical barrel: structural dissections of centriole assembly.
Dong G. (2015)
3 more