› 4n7d

X-ray diffraction
2.1Å resolution

Selenomethionine incorporated Bla g 4

Released:
DOI: 10.2210/pdb4n7d/pdb
PDB entry 4n7d coloured by chain, front view.
PDB entry 4n7d coloured by chain, side view.
PDB entry 4n7d coloured by chain, top view.
Source organism: Blattella germanica
Primary publication:
The major cockroach allergen Bla g 4 binds tyramine and octopamine.
Offermann LR, Chan SL, Osinski T, Tan YW, Chew FT, Sivaraman J, Mok YK, Minor W, Chruszcz M
Mol Immunol 60 86-94 (2014)
PMID: 24769496

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-110604 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bla g 4 allergen variant 1 Chain: A
Molecule details ›
Chain: A
Length: 176 amino acids
Theoretical weight: 20.47 KDa
Source organism: Blattella germanica
Expression system: Escherichia coli
UniProt:
  • Canonical: B7TYB2 (Residues: 1-176; Coverage: 100%)
Sequence domains: Triabin
Structure domains: Lipocalin

Ligands and Environments

2 bound ligands:
Ligand CIT 1 x CIT
Ligand GOL 2 x GOL
1 modified residue:
Ligand MSE 2 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P41212
Unit cell:
a: 60.166Å b: 60.166Å c: 124.927Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.176 0.217
Expression system: Escherichia coli

Quick links

Citations

6 review citations

New Insights into Cockroach Allergens.
Pomés et al. (2017)
5 more

2 mentions without citation

The major cockroach allergen Bla g 4 binds tyramine and octopamine.
Offermann et al. (2014)
1 more