› 4n6m

X-ray diffraction
2.9Å resolution

Crystal structure of human cystatin E/M produced in LEXSY

Released:
DOI: 10.2210/pdb4n6m/pdb
PDB entry 4n6m coloured by chain, front view.
PDB entry 4n6m coloured by chain, side view.
PDB entry 4n6m coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure and mechanism of an aspartimide-dependent peptide ligase in human legumain.
Dall E, Fegg JC, Briza P, Brandstetter H
Angew Chem Int Ed Engl 54 2917-21 (2015)
PMID: 25630877

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172420 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cystatin-M Chains: A, B
Molecule details ›
Chains: A, B
Length: 132 amino acids
Theoretical weight: 14.92 KDa
Source organism: Homo sapiens
Expression system: Leishmania tarentolae
UniProt:
  • Canonical: Q15828 (Residues: 29-149; Coverage: 100%)
Gene name: CST6
Sequence domains: Cystatin domain
Structure domains: Nuclear Transport Factor 2; Chain: A,

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21
Unit cell:
a: 39.71Å b: 82.63Å c: 43.49Å
α: 90° β: 112.81° γ: 90°
R-values:
R R work R free
0.262 0.261 0.283
Expression system: Leishmania tarentolae

Quick links

Citations

9 review citations

Macrocyclization by asparaginyl endopeptidases.
James et al. (2018)
8 more

1 mention without citation

High Molecular Weight Kininogen: A Review of the Structural Literature.
Ponczek MB. (2021)