› 4n3d

X-ray diffraction
1.34Å resolution

Crystal structure of the dimeric variant EGFP-K162Q in P61 space group

Released:
DOI: 10.2210/pdb4n3d/pdb
PDB entry 4n3d coloured by chain, front view.
PDB entry 4n3d coloured by chain, side view.
PDB entry 4n3d coloured by chain, top view.
Source organism: Aequorea victoria
Primary publication:
Three dimensional structure of the dimeric gene-engineered variant of green fluorescent protein egfp-K162Q in P61 crystal space group
Pletneva NV, V Pletnev S, Bogdanov AM, Goryacheva EA, Artemyev IV, Souslova EA, Arkhipova SF, Pletnev VZ
Rus.J.Bioorg.Chem. 40 383-389 (2014)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 242 amino acids
Theoretical weight: 27.45 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli
UniProt:
  • Canonical: P42212 (Residues: 2-232; Coverage: 97%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

3 bound ligands:
Ligand PO4 2 x PO4
Ligand K 3 x K
Ligand GOL 3 x GOL
1 modified residue:
Ligand CRO 2 x CRO

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P61
Unit cell:
a: 121.861Å b: 121.861Å c: 64.891Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.181 0.181 0.194
Expression system: Escherichia coli

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