› 4n0y

X-ray diffraction
1.75Å resolution

Structure of the Hepatitis C Envelope Glycoprotein E1 antigenic region 314-324 bound to the cross-neutralizing antibody IGH526

Released:
DOI: 10.2210/pdb4n0y/pdb
PDB entry 4n0y coloured by chain, front view.
PDB entry 4n0y coloured by chain, side view.
PDB entry 4n0y coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of Hepatitis C Virus Envelope Glycoprotein E1 Antigenic Site 314-324 in Complex with Antibody IGH526.
Kong L, Kadam RU, Giang E, Ruwona TB, Nieusma T, Culhane JC, Stanfield RL, Dawson PE, Wilson IA, Law M
J Mol Biol 427 2617-28 (2015)
PMID: 26135247

Function and Biology Details

Reactions catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Hydrolysis of four peptide bonds in the viral precursor polyprotein, commonly with Asp or Glu in the P6 position, Cys or Thr in P1 and Ser or Ala in P1'.
NTP + H(2)O = NDP + phosphate
ATP + H(2)O = ADP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-210944 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
IGH526 Heavy Chain Chain: H
Molecule details ›
Chain: H
Length: 231 amino acids
Theoretical weight: 24.57 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
IGH526 Light Chain Chain: L
Molecule details ›
Chain: L
Length: 218 amino acids
Theoretical weight: 22.85 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
Envelope glycoprotein E1 Chain: A
Molecule details ›
Chain: A
Length: 12 amino acids
Theoretical weight: 1.37 KDa
UniProt:
  • Canonical: P27958 (Residues: 314-324; Coverage: 0%)

Ligands and Environments

2 bound ligands:
Ligand GOL 7 x GOL
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P212121
Unit cell:
a: 66.326Å b: 67.628Å c: 120.719Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.179 0.21
Expression system: Homo sapiens

Quick links

Citations

14 review citations

Designing a B Cell-Based Vaccine against a Highly Variable Hepatitis C Virus.
Fuerst et al. (2017)
13 more

12 mentions without citation

Viral evasion and challenges of hepatitis C virus vaccine development.
Pierce et al. (2016)
11 more