› 4mrd

X-ray diffraction
2.55Å resolution

Crystal structure of the murine cd44 hyaluronan binding domain complex with a small molecule

Released:
DOI: 10.2210/pdb4mrd/pdb
PDB entry 4mrd coloured by chain, front view.
PDB entry 4mrd coloured by chain, side view.
PDB entry 4mrd coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Fragment-based identification of an inducible binding site on cell surface receptor CD44 for the design of protein-carbohydrate interaction inhibitors.
Liu LK, Finzel BC
J Med Chem 57 2714-25 (2014)
PMID: 24606063

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147332 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
CD44 antigen Chain: A
Molecule details ›
Chain: A
Length: 149 amino acids
Theoretical weight: 16.61 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P15379 (Residues: 23-171; Coverage: 20%)
Gene names: Cd44, Ly-24
Sequence domains: Extracellular link domain

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BDP
Carbohydrate polymer
1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: P21
Unit cell:
a: 30.62Å b: 82.28Å c: 32.52Å
α: 90° β: 117.92° γ: 90°
R-values:
R R work R free
0.19 0.187 0.245
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Carcinoma Cell Hyaluronan as a "Portable" Cancerized Prometastatic Microenvironment.
Turley et al. (2016)
1 more

5 mentions without citation

Atomic-Resolution Experimental Structural Biology and Molecular Dynamics Simulations of Hyaluronan and Its Complexes.
Guvench O. (2022)
4 more