› 4mpp

X-ray diffraction
1.5Å resolution

Crystal Structure of Benzoylformate Decarboxylase Mutant H281Y/T377P/F397T/A460I

Released:
DOI: 10.2210/pdb4mpp/pdb
PDB entry 4mpp coloured by chain, front view.
PDB entry 4mpp coloured by chain, side view.
PDB entry 4mpp coloured by chain, top view.
Source organism: Pseudomonas putida
Entry authors: Andrews FH, McLeish MJ

Function and Biology Details

Reaction catalysed: 
Benzoylformate = benzaldehyde + CO(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149084 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Benzoylformate decarboxylase Chain: A
Molecule details ›
Chain: A
Length: 536 amino acids
Theoretical weight: 57.49 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P20906 (Residues: 1-528; Coverage: 100%)
Gene name: mdlC
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand TZD 1 x TZD
4 bound ligands:
Ligand CA 2 x CA
Ligand NA 2 x NA
Ligand GOL 3 x GOL
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: I222
Unit cell:
a: 81.356Å b: 95.704Å c: 137.163Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.134 0.133 0.159
Expression system: Escherichia coli BL21(DE3)

Quick links