› 4mfg

X-ray diffraction
2Å resolution

2.0 Angstrom Resolution Crystal Structure of Putative Carbonic Anhydrase from Clostridium difficile.

Released:
DOI: 10.2210/pdb4mfg/pdb
PDB entry 4mfg coloured by chain, front view.
PDB entry 4mfg coloured by chain, side view.
PDB entry 4mfg coloured by chain, top view.
Source organism: Clostridioides difficile 630
Entry authors: Minasov G, Wawrzak Z, Kudritska M, Grimshaw S, Kwon K, Savchenko A, Anderson WF, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172675 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 168 amino acids
Theoretical weight: 18.35 KDa
Source organism: Clostridioides difficile 630
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q183I2 (Residues: 1-165; Coverage: 100%)
Gene name: CD630_27480
Sequence domains: Bacterial transferase hexapeptide (six repeats)
Structure domains: Hexapeptide repeat proteins

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand NI 4 x NI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P63
Unit cell:
a: 127.089Å b: 127.089Å c: 76.732Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.214 0.212 0.267
Expression system: Escherichia coli BL21(DE3)

Quick links