› 4mel

X-ray diffraction
2.9Å resolution

Crystal Structure of the human USP11 DUSP-UBL domains

Released:
DOI: 10.2210/pdb4mel/pdb
PDB entry 4mel coloured by chain, front view.
PDB entry 4mel coloured by chain, side view.
PDB entry 4mel coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure and catalytic regulatory function of ubiquitin specific protease 11 N-terminal and ubiquitin-like domains.
Harper S, Gratton HE, Cornaciu I, Oberer M, Scott DJ, Emsley J, Dreveny I
Biochemistry 53 2966-78 (2014)
PMID: 24724799

Function and Biology Details

Reaction catalysed: 
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-156410 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin carboxyl-terminal hydrolase 11 Chains: A, B
Molecule details ›
Chains: A, B
Length: 230 amino acids
Theoretical weight: 26.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P51784 (Residues: 67-288; Coverage: 23%)
Gene names: UHX1, USP11
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P21
Unit cell:
a: 27.725Å b: 131.982Å c: 72.029Å
α: 90° β: 96.71° γ: 90°
R-values:
R R work R free
0.234 0.231 0.297
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Recent Advances in the Discovery of Deubiquitinating Enzyme Inhibitors.
Kemp M. (2016)
5 more

2 mentions without citation

Structure and catalytic regulatory function of ubiquitin specific protease 11 N-terminal and ubiquitin-like domains.
Harper et al. (2014)
1 more