› 4mbe

X-ray diffraction
2.61Å resolution

Sac3:Sus1:Cdc31:Nup1 complex

Released:
DOI: 10.2210/pdb4mbe/pdb
PDB entry 4mbe coloured by chain, front view.
PDB entry 4mbe coloured by chain, side view.
PDB entry 4mbe coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Structural basis for binding the TREX2 complex to nuclear pores, GAL1 localisation and mRNA export.
Jani D, Valkov E, Stewart M
Nucleic Acids Res 42 6686-97 (2014)
PMID: 24705649

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-139145 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Cell division control protein 31 Chains: A, D
Molecule details ›
Chains: A, D
Length: 161 amino acids
Theoretical weight: 18.77 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P06704 (Residues: 1-161; Coverage: 100%)
Gene names: CDC31, DSK1, YOR257W
Sequence domains: EF hand
Structure domains: EF-hand
Nuclear mRNA export protein SAC3 Chains: B, E
Molecule details ›
Chains: B, E
Length: 53 amino acids
Theoretical weight: 6.62 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P46674 (Residues: 753-805; Coverage: 4%)
Gene names: LEP1, SAC3, YD8358.13, YDR159W
Sequence domains: Leucine permease transcriptional regulator helical domain
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Transcription and mRNA export factor SUS1 Chains: C, F
Molecule details ›
Chains: C, F
Length: 96 amino acids
Theoretical weight: 11.09 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6WNK7 (Residues: 1-96; Coverage: 100%)
Gene names: SUS1, YBR111W-A
Sequence domains: Transcription factor e(y)2
Structure domains: Serum Albumin; Chain A, Domain 1
Nucleoporin NUP1 Chains: G, H, X, Y
Molecule details ›
Chains: G, H, X, Y
Length: 25 amino acids
Theoretical weight: 2.89 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P20676 (Residues: 316-340; Coverage: 2%)
Gene names: NUP1, YOR098C, YOR3182C

Ligands and Environments

1 bound ligand:
Ligand CA 4 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P21
Unit cell:
a: 51.27Å b: 62.399Å c: 124.601Å
α: 90° β: 98.61° γ: 90°
R-values:
R R work R free
0.202 0.2 0.236
Expression system: Escherichia coli

Quick links

Citations

18 review citations

Transcription regulation by the Mediator complex.
Soutourina J. (2018)
17 more

3 mentions without citation

The Structure of the Nuclear Pore Complex (An Update).
Lin et al. (2019)
2 more