› 4mb1

X-ray diffraction
1.4Å resolution

The Structure of MalL mutant enzyme G202P from Bacillus subtilus

Released:
DOI: 10.2210/pdb4mb1/pdb
PDB entry 4mb1 coloured by chain, front view.
PDB entry 4mb1 coloured by chain, side view.
PDB entry 4mb1 coloured by chain, top view.
Source organism: Bacillus subtilis subsp. subtilis str. 168
Primary publication:
Change in heat capacity for enzyme catalysis determines temperature dependence of enzyme catalyzed rates.
Hobbs JK, Jiao W, Easter AD, Parker EJ, Schipper LA, Arcus VL
ACS Chem Biol 8 2388-93 (2013)
PMID: 24015933

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->6)-alpha-D-glucosidic linkages in some oligosaccharides produced from starch and glycogen by alpha-amylase, and in isomaltose.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126554 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Oligo-1,6-glucosidase 1 Chain: A
Molecule details ›
Chain: A
Length: 561 amino acids
Theoretical weight: 66.2 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O06994 (Residues: 1-561; Coverage: 100%)
Gene names: BSU34560, malL, yvdL
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand TRS 1 x TRS
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: P21
Unit cell:
a: 48.5Å b: 101.07Å c: 61.85Å
α: 90° β: 112.86° γ: 90°
R-values:
R R work R free
0.174 0.173 0.2
Expression system: Escherichia coli BL21(DE3)

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Citations

5 review citations

Enzymatic Transition States and Drug Design.
Schramm VL. (2018)
4 more

3 mentions without citation

Molecular characterization and heterologous expression of two α-glucosidases from Metschnikowia spp, both producers of honey sugars.
Garcia-Gonzalez et al. (2020)
2 more