› 4mak

X-ray diffraction
1.1Å resolution

Crystal structure of a putative ssRNA endonuclease Cas2, CRISPR adaptation protein from E.coli

Released:
DOI: 10.2210/pdb4mak/pdb
PDB entry 4mak coloured by chain, front view.
PDB entry 4mak coloured by chain, side view.
PDB entry 4mak coloured by chain, top view.
Source organism: Escherichia coli str. K-12 substr. MG1655
Entry authors: Nocek B, Skarina T, Brown G, Yakunin A, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155373 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CRISPR-associated endoribonuclease Cas2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 94 amino acids
Theoretical weight: 10.53 KDa
Source organism: Escherichia coli str. K-12 substr. MG1655
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P45956 (Residues: 1-94; Coverage: 100%)
Gene names: JW5438, b2754, cas2, ygbF
Sequence domains: CRISPR-associated protein (Cas_Cas2CT1978)
Structure domains: Alpha-Beta Plaits

Ligands and Environments

1 bound ligand:
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 30.048Å b: 66.009Å c: 69.972Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.136 0.132 0.173
Expression system: Escherichia coli BL21(DE3)

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