› 4m6g

X-ray diffraction
2.1Å resolution

Structure of the Mycobacterium tuberculosis peptidoglycan amidase Rv3717 in complex with L-Alanine-iso-D-Glutamine reaction product

Released:
DOI: 10.2210/pdb4m6g/pdb
PDB entry 4m6g coloured by chain, front view.
PDB entry 4m6g coloured by chain, side view.
PDB entry 4m6g coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Structural and biochemical analyses of Mycobacterium tuberculosis N-acetylmuramyl-L-alanine amidase Rv3717 point to a role in peptidoglycan fragment recycling.
Prigozhin DM, Mavrici D, Huizar JP, Vansell HJ, Alber T
J Biol Chem 288 31549-55 (2013)
PMID: 24019530

Function and Biology Details

Reaction catalysed: 
Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130657 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
MurNAc-LAA domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 225 amino acids
Theoretical weight: 23.43 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli
UniProt:
  • Canonical: O69684 (Residues: 20-241; Coverage: 100%)
Gene name: MT3820
Sequence domains: N-acetylmuramoyl-L-alanine amidase
Structure domains: Zn-dependent exopeptidases

Ligands and Environments

3 bound ligands:
Ligand ZN 2 x ZN
Ligand ALA 1 x ALA
Ligand ZGL 1 x ZGL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P21212
Unit cell:
a: 55.982Å b: 75.958Å c: 49.454Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.206 0.245
Expression system: Escherichia coli

Quick links

Citations

8 review citations

The cell envelope glycoconjugates of Mycobacterium tuberculosis.
Angala et al. (2014)
7 more

2 mentions without citation

Structural and biochemical analyses of Mycobacterium tuberculosis N-acetylmuramyl-L-alanine amidase Rv3717 point to a role in peptidoglycan fragment recycling.
Prigozhin et al. (2013)
1 more