› 4m58

X-ray diffraction
3.21Å resolution

Crystal Structure of an transition metal transporter

Released:
DOI: 10.2210/pdb4m58/pdb
PDB entry 4m58 coloured by chain, front view.
PDB entry 4m58 coloured by chain, side view.
PDB entry 4m58 coloured by chain, top view.
Source organism: Caldanaerobacter subterraneus subsp. tengcongensis MB4
Primary publication:
Planar substrate-binding site dictates the specificity of ECF-type nickel/cobalt transporters.
Yu Y, Zhou M, Kirsch F, Xu C, Zhang L, Wang Y, Jiang Z, Wang N, Li J, Eitinger T, Yang M
Cell Res 24 267-77 (2014)
PMID: 24366337

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185938 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cobalamin biosynthesis protein CbiM Chain: A
Molecule details ›
Chain: A
Length: 238 amino acids
Theoretical weight: 25.37 KDa
Source organism: Caldanaerobacter subterraneus subsp. tengcongensis MB4
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8R9C0 (Residues: 1-230; Coverage: 100%)
Gene names: CbiM2, TTE1695
Sequence domains: Cobalt uptake substrate-specific transmembrane region
Structure domains: Arp2/3 complex 21 kDa subunit ARPC3

Ligands and Environments

1 bound ligand:
Ligand NI 1 x NI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P6422
Unit cell:
a: 122.727Å b: 122.727Å c: 125.34Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.247 0.245 0.287
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Structural diversity of ABC transporters.
ter Beek et al. (2014)
5 more

6 mentions without citation

Elevator-type mechanisms of membrane transport.
Garaeva et al. (2020)
5 more