› 4m4w

X-ray diffraction
6.1Å resolution

Mechanistic implications for the bacterial primosome assembly of the structure of a helicase-helicase loader complex

Released:
DOI: 10.2210/pdb4m4w/pdb
PDB entry 4m4w coloured by chain, front view.
PDB entry 4m4w coloured by chain, side view.
PDB entry 4m4w coloured by chain, top view.
Source organisms:
Primary publication:
Structure of a helicase-helicase loader complex reveals insights into the mechanism of bacterial primosome assembly.
Liu B, Eliason WK, Steitz TA
Nat Commun 4 2495 (2013)
PMID: 24048025

Function and Biology Details

Reactions catalysed: 
ATP + H(2)O = ADP + phosphate
SsDNA + n NTP = ssDNA/pppN(pN)(n-1) hybrid + (n-1) diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentadecamer (preferred)
PDBe Complex ID:
PDB-CPX-139086 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Replicative DNA helicase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 454 amino acids
Theoretical weight: 50.7 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9X4C9 (Residues: 1-454; Coverage: 100%)
Gene name: dnaB
Sequence domains:
DNA primase Chains: G, H, I
Molecule details ›
Chains: G, H, I
Length: 143 amino acids
Theoretical weight: 16.8 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9X4D0 (Residues: 455-597; Coverage: 24%)
Gene name: dnaG
Primosomal protein DnaI Chains: J, K, L, M, N, O
Molecule details ›
Chains: J, K, L, M, N, O
Length: 317 amino acids
Theoretical weight: 36.99 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P06567 (Residues: 1-311; Coverage: 100%)
Gene names: BSU28980, dnaI, ytxA
Sequence domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 18 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P3121
Unit cell:
a: 229.055Å b: 229.055Å c: 364.294Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.38 0.379 0.392
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Natural supramolecular protein assemblies.
Pieters et al. (2016)
6 more

5 mentions without citation

Loading strategies of ring-shaped nucleic acid translocases and helicases.
O'Shea et al. (2014)
4 more