› 4lza

X-ray diffraction
1.84Å resolution

Crystal structure of adenine phosphoribosyltransferase from Thermoanaerobacter pseudethanolicus ATCC 33223, NYSGRC Target 029700.

Released:
DOI: 10.2210/pdb4lza/pdb
PDB entry 4lza coloured by chain, front view.
PDB entry 4lza coloured by chain, side view.
PDB entry 4lza coloured by chain, top view.
Source organism: Thermoanaerobacter pseudethanolicus ATCC 33223
Entry authors: Malashkevich VN, Bhosle R, Toro R, Hillerich B, Gizzi A, Garforth S, Kar A, Chan MK, Lafluer J, Patel H, Matikainen B, Chamala S, Lim S, Celikgil A, Villegas G, Evans B, Love J, Fiser A, Khafizov K, Seidel R, Bonanno JB, Almo SC, New York Structural Genomics Research Consortium (NYSGRC)

Function and Biology Details

Reaction catalysed: 
AMP + diphosphate = adenine + 5-phospho-alpha-D-ribose 1-diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109164 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenine phosphoribosyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 195 amino acids
Theoretical weight: 22.02 KDa
Source organism: Thermoanaerobacter pseudethanolicus ATCC 33223
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B0K969 (Residues: 1-173; Coverage: 100%)
Gene names: Teth39_1027, apt
Sequence domains: Phosphoribosyl transferase domain
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand CL 1 x CL
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P212121
Unit cell:
a: 58.95Å b: 75.914Å c: 87.522Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.203 0.228
Expression system: Escherichia coli BL21(DE3)

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