› 4lw8

X-ray diffraction
2.1Å resolution

Crystal structure of a putative epimerase from Burkholderia cenocepacia J2315

Released:
DOI: 10.2210/pdb4lw8/pdb
PDB entry 4lw8 coloured by chain, front view.
PDB entry 4lw8 coloured by chain, side view.
PDB entry 4lw8 coloured by chain, top view.
Source organism: Burkholderia cenocepacia J2315
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110034 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NAD-dependent epimerase/dehydratase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 329 amino acids
Theoretical weight: 35.5 KDa
Source organism: Burkholderia cenocepacia J2315
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B4EFL1 (Residues: 1-321; Coverage: 100%)
Gene name: BCAM1003
Sequence domains: NAD dependent epimerase/dehydratase family
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand EPE 2 x EPE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P3121
Unit cell:
a: 98.97Å b: 98.97Å c: 173.59Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.178 0.177 0.21
Expression system: Escherichia coli BL21(DE3)

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