› 4lrz

X-ray diffraction
2.32Å resolution

Crystal Structure of the E.coli DhaR(N)-DhaL complex

Released:
DOI: 10.2210/pdb4lrz/pdb
PDB entry 4lrz coloured by chain, front view.
PDB entry 4lrz coloured by chain, side view.
PDB entry 4lrz coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Coiled-coil helix rotation selects repressing or activating state of transcriptional regulator DhaR.
Shi R, McDonald L, Cygler M, Ekiel I
Structure 22 478-87 (2014)
PMID: 24440518

Function and Biology Details

Reaction catalysed: 
Phosphoenolpyruvate + glycerone = pyruvate + glycerone phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160034 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
PEP-dependent dihydroxyacetone kinase, ADP-binding subunit DhaL Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 211 amino acids
Theoretical weight: 22.67 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P76014 (Residues: 2-210; Coverage: 100%)
Gene names: JW5186, b1199, dhaL, ycgS
Sequence domains: DAK2 domain
Structure domains: Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat
PTS-dependent dihydroxyacetone kinase operon regulatory protein Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 318 amino acids
Theoretical weight: 34.89 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P76016 (Residues: 1-318; Coverage: 50%)
Gene names: JW5188, b1201, dhaR, ycgU
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ADP 4 x ADP
Ligand MG 8 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P1
Unit cell:
a: 89.766Å b: 91.5Å c: 93.808Å
α: 84.15° β: 72.42° γ: 90.01°
R-values:
R R work R free
0.202 0.2 0.247
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

The plant RWP-RK transcription factors: key regulators of nitrogen responses and of gametophyte development.
Chardin et al. (2014)
4 more

1 mention without citation

PAS domains in bacterial signal transduction.
Stuffle et al. (2021)