› 4ln8

X-ray diffraction
2.5Å resolution

The crystal structure of hemagglutinin from a h7n9 influenza virus (a/shanghai/2/2013) in complex with lstb

Released:
DOI: 10.2210/pdb4ln8/pdb
PDB entry 4ln8 coloured by chain, front view.
PDB entry 4ln8 coloured by chain, side view.
PDB entry 4ln8 coloured by chain, top view.
Source organism: Influenza A virus (A/Shanghai/02/2013(H7N9))
Primary publication:
Structural analysis of the hemagglutinin from the recent 2013 H7N9 influenza virus.
Yang H, Carney PJ, Chang JC, Villanueva JM, Stevens J
J Virol 87 12433-46 (2013)
PMID: 24027325

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero hexamer (preferred)
hetero dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-195776 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (7 distinct):
Hemagglutinin Chains: A, C, E, G, I, K
Molecule details ›
Chains: A, C, E, G, I, K
Length: 325 amino acids
Theoretical weight: 35.33 KDa
Source organism: Influenza A virus (A/Shanghai/02/2013(H7N9))
Expression system: Trichoplusia ni
UniProt:
  • Canonical: R4NN21 (Residues: 18-339; Coverage: 58%)
Gene name: HA
Sequence domains: Haemagglutinin
Structure domains:
Hemagglutinin Chains: B, D, F, H, J, L
Molecule details ›
Chains: B, D, F, H, J, L
Length: 181 amino acids
Theoretical weight: 20.88 KDa
Source organism: Influenza A virus (A/Shanghai/02/2013(H7N9))
Expression system: Trichoplusia ni
UniProt:
  • Canonical: R4NN21 (Residues: 340-517; Coverage: 32%)
Gene name: HA
Sequence domains: Haemagglutinin
Structure domains: Hemagglutinin Ectodomain; Chain B

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, GAL, SIA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, GAL, SIA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, SIA
Carbohydrate polymer
2 bound ligands:
Ligand CA 6 x CA
Ligand NAG 6 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 154.361Å b: 154.441Å c: 154.061Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.217 0.246
Expression system: Trichoplusia ni

Quick links

Citations

17 review citations

Role of receptor binding specificity in influenza A virus transmission and pathogenesis.
de Graaf et al. (2014)
16 more

5 mentions without citation

Conformations of the type-1 lacto-N-biose I unit in protein complex structures.
Fushinobu S. (2018)
4 more