› 4lmd

X-ray diffraction
1.5Å resolution

Crystal structure of the JCV large t-antigen origin binding domain

Released:
DOI: 10.2210/pdb4lmd/pdb
PDB entry 4lmd coloured by chain, front view.
PDB entry 4lmd coloured by chain, side view.
PDB entry 4lmd coloured by chain, top view.
Source organism: JC polyomavirus
Primary publication:
Insights into the initiation of JC virus DNA replication derived from the crystal structure of the T-antigen origin binding domain.
Meinke G, Phelan PJ, Kalekar R, Shin J, Archambault J, Bohm A, Bullock PA
PLoS Pathog 10 e1003966 (2014)
PMID: 24586168

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136544 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Large T antigen Chains: A, B
Molecule details ›
Chains: A, B
Length: 132 amino acids
Theoretical weight: 15.17 KDa
Source organism: JC polyomavirus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P03072 (Residues: 132-261; Coverage: 19%)
Sequence domains: Origin of replication binding protein
Structure domains: Replication Protein E1; Chain: A,

Ligands and Environments

2 bound ligands:
Ligand GOL 2 x GOL
Ligand FLC 2 x FLC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: C2
Unit cell:
a: 146.067Å b: 37.133Å c: 64.753Å
α: 90° β: 112.58° γ: 90°
R-values:
R R work R free
0.168 0.167 0.202
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Fifty Years of JC Polyomavirus: A Brief Overview and Remaining Questions.
Atkinson et al. (2020)
4 more

3 mentions without citation

Insights into the initiation of JC virus DNA replication derived from the crystal structure of the T-antigen origin binding domain.
Meinke et al. (2014)
2 more