› 4llt

X-ray diffraction
1.55Å resolution

Crystal structure of a farnesyl diphosphate synthase from Roseobacter denitrificans OCh 114, target EFI-509393, with two IPP and calcium bound in active site

Released:
DOI: 10.2210/pdb4llt/pdb
PDB entry 4llt coloured by chain, front view.
PDB entry 4llt coloured by chain, side view.
PDB entry 4llt coloured by chain, top view.
Source organism: Roseobacter denitrificans OCh 114
Entry authors: Kim J, Toro R, Bhosle R, Al Obaidi NF, Morisco LL, Wasserman SR, Sojitra S, Washington E, Scott Glenn A, Chowdhury S, Evans B, Hammonds J, Hillerich B, Love J, Seidel RD, Imker HJ, Stead M, Gerlt JA, Almo SC, Enzyme Function Initiative (EFI)

Function and Biology Details

Reaction catalysed: 
Geranyl diphosphate + isopentenyl diphosphate = diphosphate + (2E,6E)-farnesyl diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172622 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Geranyltranstransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 311 amino acids
Theoretical weight: 32.85 KDa
Source organism: Roseobacter denitrificans OCh 114
Expression system: Escherichia coli
UniProt:
  • Canonical: Q16CN9 (Residues: 1-289; Coverage: 100%)
Gene names: RD1_0549, ispA
Sequence domains: Polyprenyl synthetase
Structure domains: Farnesyl Diphosphate Synthase

Ligands and Environments

2 bound ligands:
Ligand IPE 4 x IPE
Ligand CA 6 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P21
Unit cell:
a: 50.899Å b: 83.117Å c: 71.332Å
α: 90° β: 108.95° γ: 90°
R-values:
R R work R free
0.176 0.174 0.204
Expression system: Escherichia coli

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