› 4lb5

X-ray diffraction
2Å resolution

Crystal structure of PKZ Zalpha in complex with ds(CG)6 (hexagonal form)

Released:
DOI: 10.2210/pdb4lb5/pdb
PDB entry 4lb5 coloured by chain, front view.
PDB entry 4lb5 coloured by chain, side view.
PDB entry 4lb5 coloured by chain, top view.
Source organism: Danio rerio
Primary publication:
Structural basis for Z-DNA binding and stabilization by the zebrafish Z-DNA dependent protein kinase PKZ.
de Rosa M, Zacarias S, Athanasiadis A
Nucleic Acids Res 41 9924-33 (2013)
PMID: 23975196

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117780 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Interferon-induced, double-stranded RNA-activated protein kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 72 amino acids
Theoretical weight: 8.45 KDa
Source organism: Danio rerio
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5NE14 (Residues: 5-70; Coverage: 13%)
Gene names: pkz, zgc:136657
Sequence domains: Adenosine deaminase z-alpha domain
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
5'-D(*TP*CP*GP*CP*GP*CP*GP*CP*GP*CP*GP*CP*G)-3' Chain: C
Molecule details ›
Chain: C
Length: 13 nucleotides
Theoretical weight: 3.97 KDa
Source organism: Danio rerio
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ACT 3 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P6122
Unit cell:
a: 56.86Å b: 56.86Å c: 218.77Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.192 0.188 0.224
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

7 review citations

Z-DNA and Z-RNA in human disease.
Herbert A. (2019)
6 more

8 mentions without citation

Z-DNA in the genome: from structure to disease.
Ravichandran et al. (2019)
7 more