› 4lan

X-ray diffraction
1.75Å resolution

Crystal structure of Cordyceps militaris IDCase H195A mutant

Released:
DOI: 10.2210/pdb4lan/pdb
PDB entry 4lan coloured by chain, front view.
PDB entry 4lan coloured by chain, side view.
PDB entry 4lan coloured by chain, top view.
Source organism: Cordyceps militaris CM01
Primary publication:
Crystal structures of isoorotate decarboxylases reveal a novel catalytic mechanism of 5-carboxyl-uracil decarboxylation and shed light on the search for DNA decarboxylase.
Xu S, Li W, Zhu J, Wang R, Li Z, Xu GL, Ding J
Cell Res 23 1296-309 (2013)
PMID: 23917530

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124351 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Amidohydrolase-related domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 376 amino acids
Theoretical weight: 40.65 KDa
Source organism: Cordyceps militaris CM01
Expression system: Escherichia coli
UniProt:
  • Canonical: G3J531 (Residues: 1-376; Coverage: 100%)
Gene name: CCM_01452
Sequence domains: Amidohydrolase
Structure domains: Metal-dependent hydrolases

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: C2
Unit cell:
a: 144.92Å b: 56.44Å c: 105.704Å
α: 90° β: 105.41° γ: 90°
R-values:
R R work R free
0.157 0.156 0.179
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Cancer development, progression, and therapy: an epigenetic overview.
Sarkar et al. (2013)
10 more

1 mention without citation

Crystal structures of isoorotate decarboxylases reveal a novel catalytic mechanism of 5-carboxyl-uracil decarboxylation and shed light on the search for DNA decarboxylase.
Xu et al. (2013)