› 4kxr

X-ray diffraction
2.6Å resolution

Structure of the Mycobacterium tuberculosis type VII secretion system chaperone EspG5 in complex with PE25-PPE41 dimer

Released:
DOI: 10.2210/pdb4kxr/pdb
PDB entry 4kxr coloured by chain, front view.
PDB entry 4kxr coloured by chain, side view.
PDB entry 4kxr coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Structure of the Mycobacterium tuberculosis type VII secretion system chaperone EspG5 in complex with PE25-PPE41 dimer.
Korotkova N, Freire D, Phan TH, Ummels R, Creekmore CC, Evans TJ, Wilmanns M, Bitter W, Parret AH, Houben EN, Korotkov KV
Mol Microbiol 94 367-82 (2014)
PMID: 25155747

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-125177 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
PE-PGRS family protein PE25 Chain: A
Molecule details ›
Chain: A
Length: 101 amino acids
Theoretical weight: 10.89 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: I6X486 (Residues: 1-99; Coverage: 100%)
Gene names: PE25, Rv2431c
Sequence domains: PE family
Structure domains: HP0062-like domain
PPE family protein PPE41 Chain: B
Molecule details ›
Chain: B
Length: 194 amino acids
Theoretical weight: 21.96 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q79FE1 (Residues: 1-194; Coverage: 100%)
Gene names: PPE41, Rv2430c
Sequence domains: PPE family
Structure domains: PPE superfamily
ESX-5 secretion-associated protein EspG5 Chain: C
Molecule details ›
Chain: C
Length: 300 amino acids
Theoretical weight: 32.43 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O53943 (Residues: 1-300; Coverage: 100%)
Gene names: Rv1794, espG5
Sequence domains: EspG family

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P6122
Unit cell:
a: 139.1Å b: 139.1Å c: 171.01Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.196 0.194 0.244
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

11 review citations

Secretion systems in Gram-negative bacteria: structural and mechanistic insights.
Costa et al. (2015)
10 more

10 mentions without citation

New insights into the mycobacterial PE and PPE proteins provide a framework for future research.
Ates LS. (2020)
9 more