› 4kw8

X-ray diffraction
2.46Å resolution

Crystal Structure of Green Fluorescent Protein

Released:
DOI: 10.2210/pdb4kw8/pdb
PDB entry 4kw8 coloured by chain, front view.
PDB entry 4kw8 coloured by chain, side view.
PDB entry 4kw8 coloured by chain, top view.
Source organism: Aequorea victoria
Entry authors: Barnard TJ, Yu X, Noinaj N, Taraska JW

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 239 amino acids
Theoretical weight: 27.23 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42212 (Residues: 2-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

1 bound ligand:
Ligand NI 1 x NI
1 modified residue:
Ligand CRO 1 x CRO

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P212121
Unit cell:
a: 51.623Å b: 63.021Å c: 66.347Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.24 0.152 0.225
Expression system: Escherichia coli BL21(DE3)

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