› 4kk1

X-ray diffraction
3.3Å resolution

Crystal Structure of TSC1 core domain from S. pombe

Released:
DOI: 10.2210/pdb4kk1/pdb
PDB entry 4kk1 coloured by chain, front view.
PDB entry 4kk1 coloured by chain, side view.
PDB entry 4kk1 coloured by chain, top view.
Source organism: Schizosaccharomyces pombe 972h-
Primary publication:
Crystal structure of the yeast TSC1 core domain and implications for tuberous sclerosis pathological mutations.
Sun W, Zhu YJ, Wang Z, Zhong Q, Gao F, Lou J, Gong W, Xu W
Nat Commun 4 2135 (2013)
PMID: 23857276

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170819 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tuberous sclerosis 1 protein homolog Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T
Length: 434 amino acids
Theoretical weight: 50.64 KDa
Source organism: Schizosaccharomyces pombe 972h-
Expression system: Escherichia coli
UniProt:
  • Canonical: Q09778 (Residues: 1-431; Coverage: 48%)
Gene names: SPAC22F3.13, tsc1
Sequence domains: Hamartin protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 120 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P21
Unit cell:
a: 109.321Å b: 254.829Å c: 228.472Å
α: 90° β: 99.71° γ: 90°
R-values:
R R work R free
0.19 0.186 0.268
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Regulation of mTORC1 by PI3K signaling.
Dibble et al. (2015)
2 more

1 mention without citation

Structure of the Tuberous Sclerosis Complex 2 (TSC2) N Terminus Provides Insight into Complex Assembly and Tuberous Sclerosis Pathogenesis.
Zech et al. (2016)