› 4kdc

X-ray diffraction
2.09Å resolution

Crystal Structure of UBIG

Released:
DOI: 10.2210/pdb4kdc/pdb
PDB entry 4kdc coloured by chain, front view.
PDB entry 4kdc coloured by chain, side view.
PDB entry 4kdc coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structural and biochemical studies reveal UbiG/Coq3 as a class of novel membrane-binding proteins.
Zhu Y, Wu B, Zhang X, Fan X, Niu L, Li X, Wang J, Teng M
Biochem J 470 105-14 (2015)
PMID: 26251450

Function and Biology Details

Reactions catalysed: 
S-adenosyl-L-methionine + 3-(all-trans-polyprenyl)benzene-1,2-diol = S-adenosyl-L-homocysteine + 2-methoxy-6-(all-trans-polyprenyl)phenol
S-adenosyl-L-methionine + 3-demethylubiquinone-n = S-adenosyl-L-homocysteine + ubiquinone-n
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148239 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquinone biosynthesis O-methyltransferase Chain: A
Molecule details ›
Chain: A
Length: 246 amino acids
Theoretical weight: 27.42 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P17993 (Residues: 1-240; Coverage: 100%)
Gene names: JW2226, b2232, pufX, ubiG, yfaB
Sequence domains: Methyltransferase domain
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: C2
Unit cell:
a: 119.848Å b: 58.584Å c: 40.168Å
α: 90° β: 105.26° γ: 90°
R-values:
R R work R free
0.185 0.183 0.225
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Coenzyme Q10 deficiencies: pathways in yeast and humans.
Awad et al. (2018)
3 more

4 mentions without citation

Identification of enzymes that have helminth-specific active sites and are required for Rhodoquinone-dependent metabolism as targets for new anthelmintics.
Lautens et al. (2021)
3 more