› 4k8v

X-ray diffraction
2Å resolution

Structure of cyclic GMP-AMP Synthase (cGAS)

Released:
DOI: 10.2210/pdb4k8v/pdb
PDB entry 4k8v coloured by chain, front view.
PDB entry 4k8v coloured by chain, side view.
PDB entry 4k8v coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Cyclic [G(2',5')pA(3',5')p] is the metazoan second messenger produced by DNA-activated cyclic GMP-AMP synthase.
Gao P, Ascano M, Wu Y, Barchet W, Gaffney BL, Zillinger T, Serganov AA, Liu Y, Jones RA, Hartmann G, Tuschl T, Patel DJ
Cell 153 1094-107 (2013)
PMID: 23647843

Function and Biology Details

Reaction catalysed: 
(1a) ATP + GTP = pppG-P(2'-5')A + diphosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183939 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cyclic GMP-AMP synthase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 362 amino acids
Theoretical weight: 42.51 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8C6L5 (Residues: 147-507; Coverage: 71%)
Gene names: Cgas, Mb21d1
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 86.563Å b: 84.147Å c: 124.716Å
α: 90° β: 92.74° γ: 90°
R-values:
R R work R free
0.177 0.175 0.208
Expression system: Escherichia coli

Quick links

Citations

192 review citations

Regulation and function of the cGAS-STING pathway of cytosolic DNA sensing.
Chen et al. (2016)
191 more

12 mentions without citation

Small molecules targeting the innate immune cGAS‒STING‒TBK1 signaling pathway.
Ding et al. (2020)
11 more