› 4k8l

X-ray diffraction
1.9Å resolution

Crystal structure of a putative 4-hydroxyproline epimerase/3-hydroxyproline dehydratse from the soil bacterium ochrobacterium anthropi, target efi-506495, disordered loops

Released:
DOI: 10.2210/pdb4k8l/pdb
PDB entry 4k8l coloured by chain, front view.
PDB entry 4k8l coloured by chain, side view.
PDB entry 4k8l coloured by chain, top view.
Source organism: Brucella anthropi ATCC 49188
Entry authors: Vetting MW, Toro R, Bhosle R, Al Obaidi NF, Morisco LL, Wasserman SR, Sojitra S, Stead M, Washington E, Scott Glen A, Chowdhury S, Evens B, Hammonds J, Hillerich B, Love J, Seidel RD, Imker HJ, Gerlt JA, Almo SC, Enzyme Function Initiative (EFI)

Function and Biology Details

Reaction catalysed: 
Trans-4-hydroxy-L-proline = cis-4-hydroxy-D-proline
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108144 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
4-hydroxyproline 2-epimerase 1 Chain: A
Molecule details ›
Chain: A
Length: 344 amino acids
Theoretical weight: 37.03 KDa
Source organism: Brucella anthropi ATCC 49188
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A6WW16 (Residues: 1-343; Coverage: 100%)
Gene name: Oant_0439
Sequence domains: Proline racemase
Structure domains: Diaminopimelate Epimerase; Chain A, domain 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: I222
Unit cell:
a: 77.263Å b: 78.329Å c: 114.424Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.165 0.207
Expression system: Escherichia coli BL21(DE3)

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