› 4jkg

X-ray diffraction
1.8Å resolution

Open and closed forms of mixed T1789P+R1865A and R1865A human PRP8 RNase H-like domain with bound Mg ion

Released:
DOI: 10.2210/pdb4jkg/pdb
PDB entry 4jkg coloured by chain, front view.
PDB entry 4jkg coloured by chain, side view.
PDB entry 4jkg coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A conformational switch in PRP8 mediates metal ion coordination that promotes pre-mRNA exon ligation.
Schellenberg MJ, Wu T, Ritchie DB, Fica S, Staley JP, Atta KA, LaPointe P, MacMillan AM
Nat Struct Mol Biol 20 728-34 (2013)
PMID: 23686287

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180106 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Pre-mRNA-processing-splicing factor 8 Chain: A
Molecule details ›
Chain: A
Length: 222 amino acids
Theoretical weight: 25.45 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6P2Q9 (Residues: 1769-1990; Coverage: 10%)
Gene names: PRPC8, PRPF8
Sequence domains: PRP8 domain IV core
Structure domains:
Pre-mRNA-processing-splicing factor 8 Chain: B
Molecule details ›
Chain: B
Length: 222 amino acids
Theoretical weight: 25.45 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6P2Q9 (Residues: 1769-1990; Coverage: 10%)
Gene names: PRPC8, PRPF8
Sequence domains: PRP8 domain IV core
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P212121
Unit cell:
a: 76.298Å b: 77.949Å c: 94.185Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.198 0.234
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

The noncoding RNA revolution-trashing old rules to forge new ones.
Cech et al. (2014)
7 more

1 mention without citation

A conformational switch in PRP8 mediates metal ion coordination that promotes pre-mRNA exon ligation.
Schellenberg et al. (2013)