4je3

X-ray diffraction
2.28Å resolution

An Iml3-Chl4 heterodimer links the core centromere to factors required for accurate chromosome segregation

Released:

Function and Biology Details

Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153601 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Inner kinetochore subunit IML3 Chain: A
Molecule details ›
Chain: A
Length: 245 amino acids
Theoretical weight: 28.05 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P38265 (Residues: 1-242; Coverage: 99%)
Gene names: IML3, MCM19, YBR0836, YBR107C
Inner kinetochore subunit CHL4 Chain: B
Molecule details ›
Chain: B
Length: 101 amino acids
Theoretical weight: 11.04 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P38907 (Residues: 361-458; Coverage: 21%)
Gene names: CHL4, CTF17, MCM17, YD9320A.04, YDR254W
Structure domains: Ubiquitin-like (UB roll)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: I222
Unit cell:
a: 38.483Å b: 143.916Å c: 146.909Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.183 0.211
Expression system: Escherichia coli