› 4j6u

X-ray diffraction
2.5Å resolution

Crystal Structure of Tyrosinase from Bacillus megaterium N205A mutant

Released:
DOI: 10.2210/pdb4j6u/pdb
PDB entry 4j6u coloured by chain, front view.
PDB entry 4j6u coloured by chain, side view.
PDB entry 4j6u coloured by chain, top view.
Source organism: Priestia megaterium
Primary publication:
The mechanism of copper uptake by tyrosinase from Bacillus megaterium.
Kanteev M, Goldfeder M, Chojnacki M, Adir N, Fishman A
J Biol Inorg Chem 18 895-903 (2013)
PMID: 24061559

Function and Biology Details

Reaction catalysed: 
(1a) L-tyrosine + 1/2 O(2) = L-dopa
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109772 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosinase copper-binding domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 303 amino acids
Theoretical weight: 35.21 KDa
Source organism: Priestia megaterium
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B2ZB02 (Residues: 1-297; Coverage: 100%)
Sequence domains: Common central domain of tyrosinase
Structure domains: Di-copper center containing domain from catechol oxidase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21
Unit cell:
a: 48.08Å b: 78.77Å c: 85.99Å
α: 90° β: 104.63° γ: 90°
R-values:
R R work R free
0.209 0.208 0.241
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Structure-function correlations in tyrosinases.
Kanteev et al. (2015)
2 more

1 mention without citation

Synthesis of Novel Tritopic Hydrazone Ligands: Spectroscopy, Biological Activity, DFT, and Molecular Docking Studies.
Rupa et al. (2022)