› 4j32

X-ray diffraction
1.8Å resolution

Structure of the effector - immunity system Tae4 / Tai4 from Salmonella typhimurium

Released:
DOI: 10.2210/pdb4j32/pdb
PDB entry 4j32 coloured by chain, front view.
PDB entry 4j32 coloured by chain, side view.
PDB entry 4j32 coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Primary publication:
Structural Insights into the Effector - Immunity System Tae4/Tai4 from Salmonella typhimurium.
Benz J, Reinstein J, Meinhart A
PLoS One 8 e67362 (2013)
PMID: 23826277

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187386 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cytoplasmic protein Chain: A
Molecule details ›
Chain: A
Length: 174 amino acids
Theoretical weight: 19.37 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q93IS4 (Residues: 1-161; Coverage: 100%)
Gene name: STM0277
Sequence domains: Type VI secretion system (T6SS), amidase effector protein 4
Structure domains: endopeptidase fold (from Nostoc punctiforme)
Periplasmic protein Chain: B
Molecule details ›
Chain: B
Length: 101 amino acids
Theoretical weight: 11.71 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8ZRL5 (Residues: 27-127; Coverage: 80%)
Gene name: STM0278
Sequence domains: Type VI secretion system (T6SS), amidase immunity protein
Structure domains: Four Helix Bundle (Hemerythrin (Met), subunit A)

Ligands and Environments

2 bound ligands:
Ligand FLC 2 x FLC
Ligand ETX 8 x ETX
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P6122
Unit cell:
a: 63.65Å b: 63.65Å c: 365.65Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.183 0.182 0.203
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

VgrG, Tae, Tle, and beyond: the versatile arsenal of Type VI secretion effectors.
Durand et al. (2014)
3 more

5 mentions without citation

Molecular weaponry: diverse effectors delivered by the Type VI secretion system.
Alcoforado Diniz et al. (2015)
4 more