4ios

X-ray diffraction
2.4Å resolution

Structure of phage TP901-1 RBP (ORF49) in complex with nanobody 11.

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-235410 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Baseplate protein Chains: A, B, C, H
Molecule details ›
Chains: A, B, C, H
Length: 100 amino acids
Theoretical weight: 10.55 KDa
Source organism: Lactococcus phage TP901-1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9G096 (Residues: 64-163; Coverage: 61%)
Gene name: bpp
Sequence domains: Lactophage receptor-binding protein C-terminal head domain
Structure domains: Phage tail base-plate Siphoviridae RBP, head domain
Llama nanobody 11 Chains: D, E, F, G
Molecule details ›
Chains: D, E, F, G
Length: 123 amino acids
Theoretical weight: 13.3 KDa
Source organism: Lama glama
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: R3
Unit cell:
a: 148.28Å b: 148.28Å c: 104.59Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.202 0.201 0.227
Expression system: Escherichia coli