› 4ibk

X-ray diffraction
1.85Å resolution

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170140 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polymerase cofactor VP35 Chains: A, B
Molecule details ›
Chains: A, B
Length: 129 amino acids
Theoretical weight: 14.21 KDa
Source organism: Ebola virus - Mayinga, Zaire, 1976
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q05127 (Residues: 215-340; Coverage: 37%)
Gene name: VP35
Sequence domains: Filoviridae VP35
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand 1DE 2 x 1DE
Ligand GOL 1 x GOL
Ligand DMS 2 x DMS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 51.222Å b: 65.689Å c: 72.831Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.192 0.241
Expression system: Escherichia coli BL21(DE3)

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Citations

9 review citations

Perspectives towards antiviral drug discovery against Ebola virus.
Mirza et al. (2019)
8 more

6 mentions without citation

In silico derived small molecules bind the filovirus VP35 protein and inhibit its polymerase cofactor activity.
Brown et al. (2014)
5 more