› 4i6s

X-ray diffraction
1.54Å resolution

Structure of RSL mutant W76A in complex with L-fucose

Released:
DOI: 10.2210/pdb4i6s/pdb
PDB entry 4i6s coloured by chain, front view.
PDB entry 4i6s coloured by chain, side view.
PDB entry 4i6s coloured by chain, top view.
Source organism: Ralstonia solanacearum
Primary publication:
Reduction of lectin valency drastically changes glycolipid dynamics in membranes but not surface avidity.
Arnaud J, Claudinon J, Tröndle K, Trovaslet M, Larson G, Thomas A, Varrot A, Römer W, Imberty A, Audfray A
ACS Chem Biol 8 1918-24 (2013)
PMID: 23855446

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-241374 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative fucose-binding lectin protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 91 amino acids
Theoretical weight: 9.75 KDa
Source organism: Ralstonia solanacearum
Expression system: Escherichia coli
Structure domains: Lipocalin

Ligands and Environments

4 bound ligands:
Ligand FUC 5 x FUC
Ligand FUL 4 x FUL
Ligand EDO 1 x EDO
Ligand PG4 2 x PG4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: I4122
Unit cell:
a: 103.765Å b: 103.765Å c: 100.834Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.149 0.148 0.172
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Recent advances in engineering polyvalent biological interactions.
Varner et al. (2015)
5 more

1 mention without citation

Influence of Trp flipping on carbohydrate binding in lectins. An example on Aleuria aurantia lectin AAL.
Houser et al. (2017)